Evaluation of Aspergillus Galactomannan Lateral Flow Assay on Serum and Bronchoalveolar Lavage Specimens
نویسندگان
چکیده
Background : Detection of biomarkers, such as galactomannan (GM), has proven to be great significance in early recognition invasive aspergillosis (IA). The aim our study was evaluate the lateral flow assay (LFA) for detection GM on serum and bronchoalveolar lavage (BAL) samples previously positive by enzyme-linked immunosorbent (ELISA). Methods: performed BAL obtained from patients with suspected IA period February 2019 January 2020, which were ELISA (Platelia Aspergillus Ag, Biorad, Hercules, USA). Samples then tested LFA ( Galactomannan LFA, IMMY, Oklahoma, USA) test line intensity visually read 1+, 2+, 3+, or 4+. Results: A total 45 and/or 41 patients; 25 (55,6 %) 20 (44,4 samples. showed a result 39 out (86,7%) samples; 22/25 (88.0 17/20 (85.0 positive. In samples, low 1+ significantly more frequent optical density index (ODI) < 1.0 (p=0.0002). Three high ODI (>4.0) had when LFA. Conclusions: Results are comparable ELISA. Since lines found ODI, this potentially makes superior sample at least visual not automated reading is done.
منابع مشابه
The Diagnosis of Invasive and Noninvasive Pulmonary Aspergillosis by Serum and Bronchoalveolar Lavage Fluid Galactomannan Assay
The incidence and mortality of invasive pulmonary aspergillosis (IPA) are rising, particularly in critically ill patients and patients with severe chronic obstructive pulmonary disease (COPD). Noninvasive aspergillosis occurring in these patients requires special attention because of the possibility of developing subsequent IPA, given the poor health and worsened immune state of these patients....
متن کاملPlasmalyte as a cause of false-positive results for Aspergillus galactomannan in bronchoalveolar lavage fluid.
The detection of galactomannan (GM) in the serum of immunocompromised patients is widely used for the early diagnosis of invasive aspergillosis (6). The test may also be useful when applied to bronchoalveolar lavage (BAL) fluid specimens for clinical diagnosis (5), though not FDA approved for this use. One important limiting factor for GM testing is the potential for false-positive results. Mos...
متن کاملPerformance of galactomannan, beta-d-glucan, Aspergillus lateral-flow device, conventional culture, and PCR tests with bronchoalveolar lavage fluid for diagnosis of invasive pulmonary aspergillosis.
Galactomannan detection in bronchoalveolar lavage (BAL) fluid samples (GM test) is currently considered the gold standard test for diagnosing invasive pulmonary aspergillosis (IPA). The limitations, however, are the various turnaround times and availability of testing. We compared the performance of GM testing with that of conventional culture, an Aspergillus lateral-flow-device (LFD) test, a b...
متن کاملComparison of serum and bronchoalveolar lavage galactomannan in diagnosing invasive aspergillosis in solid-organ transplant recipients.
OBJECTIVES This study sought to compare the sensitivities of serum galactomannan and bronchoalveolar lavage galactomannan in diagnosing invasive aspergillosis in solid-organ transplant recipients (lung and heart). MATERIALS AND METHODS This study took place in the lung transplant center of the National Research Institute for Tuberculosis and Lung Disease. All patients with clinical and radiol...
متن کاملPerformance characteristics of the platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid.
We have evaluated the Platelia Aspergillus enzyme immunoassay for detection of galactomannan in bronchoalveolar lavage (BAL) specimens in solid organ transplant patients with aspergillosis. The precision and reproducibility in serum or BAL to which galactomannan was added were similar. Sensitivity was 81.8% in patients with aspergillosis, and specificity was 95.8% in lung transplant patients wh...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: croatian journal of infection
سال: 2022
ISSN: ['1848-7769', '1331-2820']
DOI: https://doi.org/10.37797/ig.41.2.1